Intracellular localization of samarium in the lactating mammary gland cells: Ultrastructural and microanalytical study
Identifieur interne : 000420 ( Main/Exploration ); précédent : 000419; suivant : 000421Intracellular localization of samarium in the lactating mammary gland cells: Ultrastructural and microanalytical study
Auteurs : Ayadi Ahlem [Tunisie] ; Maghraoui Samira [Tunisie] ; Audinot Jean-Nicolas [Luxembourg (pays)] ; Jaafoura Mohamed-Habib [Tunisie] ; Migeon Henri-Noël [Luxembourg (pays)] ; El Hili Ali [Tunisie] ; Tekaya Leila [Tunisie]Source :
- Microscopy Research and Technique [ 1059-910X ] ; 2012-04.
English descriptors
Abstract
The frequent use of some rare earths in the medical and industrial domains make us worry about their intracellular behavior into the body. Reason for which we have investigated the subcellular localization of one of these elements, the samarium, in the mammary gland of lactating female wistar rats using two very sensitive methods of observation and microanalysis, the transmission electron microscopy and the secondary ion mass spectrometry. The ultrastructural study showed the presence of electron dense deposits in the lactating mammary glandular epithelial cell lysosomes of the samarium‐treated rats, but no loaded lysosomes were observed in those of control rats. The microanalytical study allowed both the identification of the chemical species present in those deposits as samarium isotopes (152Sm+) and the cartography of its distribution. Our results confirm the previous ones showing that lysosomes of the glandular epithelial cells are the site of the intracellular concentration of foreign elements such as gallium. The intralysosomal deposits observed in the mammary glandular cells of the samarium‐treated rats are similar in their form and density to those observed with the same element in other varieties of cells, such as liver, bone marrow, and spleen cells. Our ultrastructural and microanalytical results and those obtained in previous studies allow deducing that the intralysosomal deposits are very probably composed of an insoluble samarium phosphate salt. © 2011 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jemt.21076
Affiliations:
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Reason for which we have investigated the subcellular localization of one of these elements, the samarium, in the mammary gland of lactating female wistar rats using two very sensitive methods of observation and microanalysis, the transmission electron microscopy and the secondary ion mass spectrometry. The ultrastructural study showed the presence of electron dense deposits in the lactating mammary glandular epithelial cell lysosomes of the samarium‐treated rats, but no loaded lysosomes were observed in those of control rats. The microanalytical study allowed both the identification of the chemical species present in those deposits as samarium isotopes (152Sm+) and the cartography of its distribution. Our results confirm the previous ones showing that lysosomes of the glandular epithelial cells are the site of the intracellular concentration of foreign elements such as gallium. The intralysosomal deposits observed in the mammary glandular cells of the samarium‐treated rats are similar in their form and density to those observed with the same element in other varieties of cells, such as liver, bone marrow, and spleen cells. Our ultrastructural and microanalytical results and those obtained in previous studies allow deducing that the intralysosomal deposits are very probably composed of an insoluble samarium phosphate salt. © 2011 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Luxembourg (pays)</li><li>Tunisie</li></country><region><li>Gouvernorat de Tunis</li></region><settlement><li>Tunis</li></settlement></list><tree><country name="Tunisie"><region name="Gouvernorat de Tunis"><name sortKey="Ahlem, Ayadi" sort="Ahlem, Ayadi" uniqKey="Ahlem A" first="Ayadi" last="Ahlem">Ayadi Ahlem</name></region><name sortKey="Ali, El Hili" sort="Ali, El Hili" uniqKey="Ali E" first="El Hili" last="Ali">El Hili Ali</name><name sortKey="Leila, Tekaya" sort="Leila, Tekaya" uniqKey="Leila T" first="Tekaya" last="Leila">Tekaya Leila</name><name sortKey="Mohamed Abib, Jaafoura" sort="Mohamed Abib, Jaafoura" uniqKey="Mohamed Abib J" first="Jaafoura" last="Mohamed-Habib">Jaafoura Mohamed-Habib</name><name sortKey="Samira, Maghraoui" sort="Samira, Maghraoui" uniqKey="Samira M" first="Maghraoui" last="Samira">Maghraoui Samira</name></country><country name="Luxembourg (pays)"><noRegion><name sortKey="Jean Icolas, Audinot" sort="Jean Icolas, Audinot" uniqKey="Jean Icolas A" first="Audinot" last="Jean-Nicolas">Audinot Jean-Nicolas</name></noRegion><name sortKey="Henri Oel, Migeon" sort="Henri Oel, Migeon" uniqKey="Henri Oel M" first="Migeon" last="Henri-Noël">Migeon Henri-Noël</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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